Cy 3 low with dyesaver2

This forum is dedicated to the use and analysis of microarrays and other aspects of chip technology.

Moderators: mdfenko, Abhijeet Bakre

Cy 3 low with dyesaver2

Postby tmastro » Oct 10 2007 7:45 pm

I have heard that Cy 3 has a stronger signal than Cy 5. This does not seem to be true when using dyesaver2 on our slides. When we use dyesaver2 the Cy 5 signal is preserved well but the Cy 3 signal drops off. Does anyone know if there something about dyesaver2 that does not protect Cy 3 so that when it is exposed to air/light the dye is deteriorating? Has anyone else experienced this problem?
Posts: 1
Joined: Oct 10 2007 7:26 pm

Postby spielerei » Aug 20 2008 1:47 pm

hi tmastro,

I tried the dyesaver reagent once, and I didn't see a positive affect for the Cy5, and the background of the Cy3 signal went up so the signal / bkgr values dropped,
didn't like it at all so I stopped using dyesavers,
Without "spielerei" there is no science, always follow your instinct first
Posts: 4
Joined: Aug 20 2008 12:55 pm

Return to Microarrays

Who is online

Users browsing this forum: No registered users and 1 guest