Luminex:beads

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Luminex:beads

Postby elix71 » Aug 26 2008 12:32 pm

Hello,
I am developing protocols of immunoassays with my antigens on Luninex technology in single Plex and I would like ask some technical suggestion about beads aggregates forming during the incubation:
I know that is always recommended to filter sera before start the incubation but sometime the sera amount is so small and it's colud be difficult !
Well, Do you Know if centrifuge the sera is recommended? I noticed a big good difference with this procedure but actually I don’t know if could be some differences about the amount of total IgG express in FI.
Thanks for your cooperation.
elix71
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