Beads aggregates

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Beads aggregates

Postby elix71 » Mar 06 2009 10:51 am

I am developing protocols of immunoassays with my antigens on Luninex technology in single Plex and I would like ask some technical suggestion about beads aggregates forming during the incubation:
I know that is always recommended to filter sera before start the incubation but sometime the sera amount is so small and it's colud be difficult !
Well,in the rabbit serum the problem is bigger and reading plates is really difficult. I have tried to increase the beads number but the results are the same. Can you suggest how to solve the problem?

Thanks for your cooperation
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