Guidlines for species specific probe design

This forum is dedicated to the use and analysis of microarrays and other aspects of chip technology.

Moderators: mdfenko, Abhijeet Bakre

Guidlines for species specific probe design

Postby brendanconneely » Jan 26 2010 6:38 pm

Hello,
Im trying to design a panel of species specific probes for the identification of 13 species. The probes will be employed on a reverse line blot (which is very similar to a micro array only larger and cheaper). I am using the internal transcribed spacer 2 (ITS2) region of the rDNA array as a diagnostic target. My problem is I dont have any guidelines on the probe design only that they should be between 18-30 bases in length. My main question is how many bases in my probe sequence need to be need to be different from other species ITS2 sequence to make that probe species specific, and also where should these differences occurr in my probe sequence, the outside of the sequence or in the middle ?
Any help would be greatly appreciated
brendanconneely
technician-in-training
technician-in-training
 
Posts: 11
Joined: May 14 2009 9:58 am

Return to Microarrays

Who is online

Users browsing this forum: No registered users and 1 guest

cron