Why did those cells all died?

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Why did those cells all died?

Postby 2444256703 » Aug 11 2015 9:23 am

I cultured the vascular smooth muscle cells with 20% medium, and changed medium after 3-4 days when cell were out, but two days later the cells were all dead, and I do not know what caused it. Anyone knows? Appreciate your explanation!
Last edited by mdfenko on Aug 11 2015 1:05 pm, edited 1 time in total.
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Re: Why did those cells all died?

Postby Joannajone » Jun 24 2016 9:29 am

Human vascular smooth muscle cells (VSMCs) in culture are an important tool in understanding how VSMCs function and contribute to vessel wall contraction as well as disease. These VSMCs are heterogeneous and vary in size, shape, and proliferative capacity depending on the disease state and location of the vessel of origin. An important feature of VSMCs in culture is that they lose many of their in vivo contractile properties and so model disease-associated VSMCs in the vessel wall rather than a non-proliferative contractile cell.
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