Cell death following lentiviral transduction

Forum for prokaryotic and eukaryotic culture, cell culture related assays (e.g. CTL, cytokine release, B cell culture), cell lines/primary culture, reagents, etc.

Cell death following lentiviral transduction

Postby laleh » Aug 24 2016 2:29 am

Hi

We are trying to transduce mammalian cells with lentiviral vector, we produce the particles in HEK293T and we have used both the concentrated virus and the harvested cell culture medium in different dilutions with or without different concentrations of polyberen. We replace the virus containing medium with fresh media 4-6 hours post transduction. As soon as the virus is added to cells their morphology changes and massive cell death occurs in 24 hours. We can see the GFP expression from the virus in low percentage of the remaining cells.
I was wondering if anybody else has had the same problem.

Thanks
laleh
supertech
supertech
 
Posts: 59
Joined: Jul 18 2009 4:27 am

Return to Cell Culture

Who is online

Users browsing this forum: No registered users and 1 guest