fibroblast cell culture from adult skin

Forum for prokaryotic and eukaryotic culture, cell culture related assays (e.g. CTL, cytokine release, B cell culture), cell lines/primary culture, reagents, etc.

fibroblast cell culture from adult skin

Postby laleh » Nov 02 2016 11:21 am

Dear all

I need to develop an skin primary cell culture for further analysis which may take up to 1 month. I have heard different opinions from my colleagues regarding the duration which we can passage skin fibroblast and keratinocytes (isolated from adults). I was wondering if anyone has had an experiment with these cell types.

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