LP9 cells

Forum for prokaryotic and eukaryotic culture, cell culture related assays (e.g. CTL, cytokine release, B cell culture), cell lines/primary culture, reagents, etc.

LP9 cells

Postby LP9 cells » Feb 27 2017 2:26 am

I have been cultivating LP9 cells (human peritoneal mesothelial cells) for over a year now, but they never reach confluence. They are newly purchased, mycoplasma-free cells, and I've tried cultivating them on different plastics and coating the plates with fibronectin. I use new EGF in the medium and I even increased its concentration to encourage growth, but the LP9 cells still aren't confluent. How can I solve this problem? Any help would be greatly appreciated!
LP9 cells
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