Free DNA from culture media

Forum for prokaryotic and eukaryotic culture, cell culture related assays (e.g. CTL, cytokine release, B cell culture), cell lines/primary culture, reagents, etc.

Free DNA from culture media

Postby ricardopella » Jul 20 2017 4:09 pm

How can I safely eliminate cell-free DNA from my culture media? Can I use DNAase without damaging my cells in culture?
Posts: 1
Joined: Jul 20 2017 3:35 pm

Re: Free DNA from culture media

Postby relaxin » Jul 22 2017 2:28 pm

Free DNA in culture media comes from fetal calf serum. Can your cell grow in serum-free medium?

DNase should not damage cells. But it will be difficult to determine how much DNase you need to clear the free DNA in the medium.
Retired academic researcher. Mention of a specific product does not imply my endorsement of the product. No conflict of interest or guarantee to work on the advice given. Do as I say, not as I do. Not liable to the loss of your valuable samples.
PI of Posters
PI of Posters
Posts: 7190
Joined: Jan 11 2006 5:40 pm
Location: Mauna Kea

Return to Cell Culture

Who is online

Users browsing this forum: No registered users and 2 guests