how to quantify GFP using qPCR?

Use this category for questions related to various quantitative PCR methods, including real time PCR, reverse transcription (RT)/PCR, qPCR, etc. For general PCR methods, continue to use the DNA/General PCR forum

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how to quantify GFP using qPCR?

Postby iceqube » Dec 04 2016 10:17 pm

How to Quantify green fluorescent protein fluorescence using real-time PCR thermal cycler?
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Re: how to quantify GFP using qPCR?

Postby lisajcantrell » Dec 31 2016 9:44 am

The potential for using real-time qPCR amplification of human repeats to accurately quantify small numbers of cells in vivo in scientific investigations has been known for more than 20 years but this technique has had limited value due to high background signals from human contamination and the challenges of consistent DNA extraction across different tissue types.
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