qPCR reaction with probe self-dimer

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qPCR reaction with probe self-dimer

Postby namtran93 » May 10 2017 9:51 am

Hello everyone,

I am trying to optimize a qPCR reaction. Unluckily, I realize that my probe form a self-dimer structure which impair the signal of amplification. How can I improve my qPCR reaction? I hope that someone can help me. :cry: :cry: :cry: :cry:
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Joined: May 10 2017 9:47 am

Re: qPCR reaction with probe self-dimer

Postby DrShabba » Aug 08 2017 1:09 pm

How did you discover the self-dimer, are you sure that is impairing the reaction?

What type of fluorophore and quencher are you using?

Redesign your probe if possible - if you can't change the sequence then try ordering from a different company
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Joined: Jul 19 2017 10:54 am

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