How to retain BFP fluorescence in immunostainig?

Use for discussion of various microscopy and imaging techniques, such as phase, fluorescence and confocal microscopy, electron microscopy, and various other imaging techniques.

Moderator: r.rosati

How to retain BFP fluorescence in immunostainig?

Postby shubhamk » Jun 27 2017 10:27 am

I have to visualize microtubules with my protein tagged with BFP fluorophore. I transfect the cells and do immunostaining but I loose the BFP signal. What is the best fixation and permeabilization method I can use to retain the BFP signal?
shubhamk
newcomer
newcomer
 
Posts: 1
Joined: Jun 27 2017 4:17 am

Return to Microscopy and Imaging Techniques

Who is online

Users browsing this forum: No registered users and 1 guest

cron