Stereo microscope imaging problem

Use for discussion of various microscopy and imaging techniques, such as phase, fluorescence and confocal microscopy, electron microscopy, and various other imaging techniques.

Moderator: r.rosati

Stereo microscope imaging problem

Postby Archana G N » Mar 20 2018 7:00 am

Hello everyone,
I am using stereo microscope to image wound created on an animal model and tracking the wound healing over time by taking images every hour in stereo microscope at 2x magnification. To see the percentage of area healed , i draw the line along the inner diameter of the wound for the every hour image using Imagej software.
As assumed the area should decrease with time(as the wound is healing)
But even after many repetitions i am finding that the area of wound is not linearly decreasing. Sometimes they just just decrease and increase(which is impossible!).
So please provide me with a solution.
Also i manually draw over the wound border to calculate the area, which makes it even more difficult since the wound borders are not clearly defined. can someone please suggest me any other software to do so easily?
Archana G N
newcomer
newcomer
 
Posts: 1
Joined: Mar 20 2018 6:47 am

Return to Microscopy and Imaging Techniques

Who is online

Users browsing this forum: No registered users and 1 guest