What is the best nuclear stain for microscopy?

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What is the best nuclear stain for microscopy?

Postby IFqs » Apr 07 2009 3:20 pm

I find that DAPI leaks out of the nucleus over time, and is very variable in intensity. Can anyone suggest an alternative? Is Hoechst any better?
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Re: What is the best nuclear stain for microscopy?

Postby Gnapponico » Jun 01 2009 4:17 pm

Hi! it depends on your microscopy system...
In my lab we use Propidium Iodide or Ethidium Bromide (excitation on 488 nm, they can be detected with 562-588 nm band pass filter) for nucleus staining in confocal microscopy...
but you can also use:
Acridine orange (ex. 502 em. 526)
TOTO3 (ex. 642 em. 661)




(PS: sometimes Eosin can be used as a nuclear stain and as an economical substitute for nuclear dyes...but you need a IR or near IR laser...)
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Re: What is the best nuclear stain for microscopy?

Postby tibhar » Jan 20 2011 8:54 am

Fix longer than 10min with 4% paraformaldhyde, or just formaldhyde?
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