Image J to score DAPI staining

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Image J to score DAPI staining

Postby wangleipurdue » Jun 17 2010 3:31 am

Dear all,

I got another problem for Image J. My boos asked me to score TUNEL staining for apoptosis, that means I need to score almost 1000 DAPI (nucleus) to see among them how many TUNEL positive cell according to her. That is a lot of scoring under microscope.

I wonder whether I can take images and use Image J to score DAPI (I just need to score TUNEL).

Is there anyone knows how to use Image J to score DAPI?

Thank you.

Lei
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Re: Image J to score DAPI staining

Postby ahem » Aug 05 2010 1:39 pm

hi,
if by score you mean to count, you can use the cell counter plugin. you can download it from the ImageJ website.
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