Looking for suitable GFP variant for gram-negative bacteria

Use for discussion of various microscopy and imaging techniques, such as phase, fluorescence and confocal microscopy, electron microscopy, and various other imaging techniques.

Moderator: r.rosati

Looking for suitable GFP variant for gram-negative bacteria

Postby WhenIsLunch » Jun 02 2011 6:21 pm

Hello everyone,

I'm going to be doing confocal microscopy on a bacterial biofilm. I want to make some transcriptional reporters by fusing the promoters of genes with a GFP reporter. I'm looking for a GFP variant that is bacterial codon optimized, has good quantum yield, and is destabilized in gram-negative bacteria (the shorter the half-life, the better). Any suggestions?
WhenIsLunch
newcomer
newcomer
 
Posts: 1
Joined: Jun 02 2011 6:13 pm

Re: Looking for suitable GFP variant for gram-negative bacte

Postby CrowSan » Jun 07 2011 3:24 pm

Hi you might want to check out this paper: "Tuning the strength of a bacterial N-end rule degradation signal"
Wang et al, JBC. 283 (36). pg. 24600-24607. 2008
It's free on the web and they seem to use bacterial, destabilised GFP. They may even give you some.
CrSn
CrowSan
PI of Posters
PI of Posters
 
Posts: 515
Joined: May 17 2010 12:13 pm


Return to Microscopy and Imaging Techniques

Who is online

Users browsing this forum: No registered users and 2 guests