4% PFA Fixation time for GFP?

Use for discussion of various microscopy and imaging techniques, such as phase, fluorescence and confocal microscopy, electron microscopy, and various other imaging techniques.

Moderator: r.rosati

4% PFA Fixation time for GFP?

Postby hellohippo9 » Mar 14 2013 10:16 am

Is 4% PFA fixed 1 hour in 4 degree enough for cultured cells (primary osteoblast). My target protein is a GFP-fused protein.

Thanks a lot!
hellohippo9
newcomer
newcomer
 
Posts: 1
Joined: Mar 14 2013 10:09 am

Return to Microscopy and Imaging Techniques

Who is online

Users browsing this forum: No registered users and 1 guest