How to improve Q-FISH

Use for discussion of various microscopy and imaging techniques, such as phase, fluorescence and confocal microscopy, electron microscopy, and various other imaging techniques.

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How to improve Q-FISH

Postby mhviegas » Apr 21 2013 7:53 am

Dear forum members. I have recently entered a lab that performs qFISH for telomere analysis basically following the protocol described by Lansdorp et al (1996). However, I realize that the PNA probe (ccctaa)3 gives quite a significant background. I wonder if any of you have experience working with PNA probes and know how to improve the signal-to-noise ratio.
Many thanks in advance!
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