Fluorescence lump formation in an protein sandwich assay

Use for discussion of various microscopy and imaging techniques, such as phase, fluorescence and confocal microscopy, electron microscopy, and various other imaging techniques.

Moderator: r.rosati

Fluorescence lump formation in an protein sandwich assay

Postby aman_1183 » May 17 2013 11:29 am

Hi All,

I am new to fluorescence microscopy. I am trying to detect a sandwich assay using FITC labeelled streptavidin. What I did was, I first put the anchoring antibody followed by the antigen and then another antibody which was biotinylated. Over this biotinylated antibody, I put FITC labeelled streptavidin. I area of intrest get illuminated as expected but along with the illuminated surface I also see alot of fluroscence lump
f sample_positive area.tif
. Could some one please tell me what could be the cause for it and how to avoid it.

You do not have the required permissions to view the files attached to this post.
Posts: 4
Joined: Feb 09 2010 1:09 pm

Return to Microscopy and Imaging Techniques

Who is online

Users browsing this forum: No registered users and 1 guest