Stereology imaging after confocal microscopy

Use for discussion of various microscopy and imaging techniques, such as phase, fluorescence and confocal microscopy, electron microscopy, and various other imaging techniques.

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Stereology imaging after confocal microscopy

Postby anikapl » Mar 09 2015 6:56 am

Hi! I'm using stack images after confocal microscopy to measure 2 types of cells in mouse brain after status epilepticus. PV cells (red channel) and VVA cells (green channel). I also want to measure their co-localization. Which is the best way to do the measurement in stereology? Should I use the stacks with all the channels and make all the three measurements there? Or should I use each channel separately?

I really need some advice here. Thank you in advance.
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