Storage of genetic material in a magnetic field

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Storage of genetic material in a magnetic field

Postby Yurikov » Jun 07 2018 2:27 pm

In this article we consider a version according to which the Earth's magnetic field has a direct effect on the probability of reprogramming DNA chains, and justifies the assumption that in a strong magnetic field the decay of isolated DNA molecules completely ceases, which can be of practical importance for the long-term storage of genetic material.
The basis for this assumption are the results of numerous studies of the effects of hypomagnetic and hyper magnetic fields on living organisms, performed primarily in the interests of space medicine.
The analysis of the various consequences of the negative influence of the weakened geomagnetic field on living organisms shows that their basis is genetic disorders. Being in a hypomagnetic field causes atypical growth of cells and tissues, the appearance of mutant cell forms, causes effects similar to the cellular response to DNA damage, up to the phenomenon of programmed cell death.
In very strong magnetic, or in other words, hypermagnetic fields, in the aspect of reprogramming the DNA, there are opposite tendencies. Analysis of experimental data suggests that under these conditions the process of differentiation of stem cells, their transformation into cells of various tissues is blocked (including the growth of cancer cells also stops). At the same time, cells of already existing tissues continue to be freely shared. The process of memorization is based on reprogramming the DNA of neurons, and in strong magnetic fields this process is suppressed.
The above conclusions can be illustrated by the following examples. As a result of studies conducted during the time of the existence of the USSR, it has been established that mutant forms of cells appear in microorganisms that were in conditions without a geomagnetic field. Without a geomagnetic field, the mice survived to 4 to 12 months. In the first generation, females crossed with males of the same group brought normal offspring. In the second generation, premature miscarriages and cannibalism were noted. And by the fourth generation the reproduction of mice stopped.
There were other signs of abnormal development of animals in conditions without a magnetic field. So, at an early age the mice became sluggish and inactive, they lay on their backs for a long time, and about 14% of them had baldness from the head to the back half. By the age of six months, most of the animals had died.
When a thorough histological analysis of the organs of mice and their skin was carried out, then cancerous formations were found in different parts of the body. The nuclei of the hepatic tissue of mice that were contained without a geomagnetic field changed. The kidneys were also greatly altered, they became multi-chambered and cysts appeared. In those experimental mice that died suddenly, the bladder was filled with urine and white sediment. He had a mucous membrane with polyps and septa [1].
Incubation of eggs under hypogeomagnetic conditions disrupts the development of chick embryos, many of them die, and hatched chicks have dystrophic changes in internal organs, paresis of wings and legs, etc. [2], Chickens in hypomagnetic conditions stop bringing eggs [3].
Screening of the geomagnetic field 600 times leads to inhibition of the growth of rabbits, a decrease in their motor activity, the development of dystrophic changes in the liver, myocardium, stomach, intestines, a decrease in the activity of key enzymes of the tricarboxylic acid cycle and the pentose phosphate cycle [4].
In conditions of a shielded geomagnetic field on the cells of the HeLa and VH-10 lines, the P-53 protein and the signaling pathways mediated by it are actively involved in adapting the cell to the conditions of the hypogeomagnetic field. The observed effect on the parameters studied was similar to the cellular response to DNA damage [5].
In the work of E. Lushnikova. and others, studies of the effect of hypogeomagnetic fields on mice, in particular, on their cardiovascular system, are described. "The final stages of the morphofunctional reconstruction of cardiomyocytes resemble the phenomenon of programmed cell death. 10 days of stay of mice in such cells led them to death " [6].
All the embryos of the Japanese quail, which were in hypomagnetic conditions, without exception, were found to have some anomalies in the development of the cardiovascular system. One of the embryos had a pathology of the caudal part of the trunk - cleavage into two [7].
In a shielded chamber in which the geomagnetic field was attenuated by approximately 10,000 times, two heads appeared in the larvae of the Japanese Triton Cynops pyrrhogaster, the curvature of the spine, unformed eyes, and delayed or blocked development [8]
Pregnant mice at the zygote stage, placed in a "zero" magnetic field, lost the ability to give birth, although their embryos developed before the blastocyst stage [9].
Near to zero magnetic fields adversely affected the growth and development of small brown planthopper (abbr. SBPH) and brown planthopper (abbr. BPH), especially strongly affecting reproduction [10].
Hypomagnetic conditions cause and violations of the kinetics of protein assembly:
HMF suppresses gene expression associated with cell migration and cytoskeleton assembly in human neuroblastoma cells (SH-SY5Y cell line). During the exposure to HMF, the disordered kinetics of actin assembly in vitro was observed, as evidenced by the presence of granules and products with cells [11].
Elimination of the geomagnetic field causes disturbances in the assembly of tubulin [12] [13].
The hypomagnetic field suppresses the development of the organism in the early stages:
Numerous experiments with seedlings of various plant species placed in a weak magnetic field have shown that the growth of their primary roots is inhibited in the early stages of germination in comparison with the control. In plant cells exposed to a weak magnetic field, the functional activity of the genome decreases in the early period before replication [14].
Proliferative activity and multiplication of cells in the meristem of plant roots decrease in a weak magnetic field [15].
Under conditions of ZMF, the processes of denaturation and degradation of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in human serum proceed faster [16].
Hypomagnetic conditions cause violations of genetic memory:
Serious but reversible disabilities in learning ability and memory occur in fruit flies in the hypomagnetic field, which is reflected in many subsequent generations [17].
The following examples demonstrate that under conditions of very strong magnetic fields, the processes associated with reprogramming the DNA are completely blocked. These studies were conducted in the sixties in the United States.
In mice grown in a magnetic field whose intensity was 5000 times greater than the intensity of the Earth's magnetic field and varied with time and place from 0.03 to 0.4 oersted, an increase in the number of white blood cells, the disappearance of cancerous formations (tumors) and weakening of the sexual instinct. The offspring born in this magnetic field died within a few days. Male mice that did not reach puberty, when exposed to such a magnetic field, also died. Adult mice-males survived under the same conditions.
In a magnetic field of 140,000 oersteds, the rate of cell division in eggs of sea urchins significantly decreases, and at the same time it does not significantly affect the motor activity of mice or the period of life of fruit flies. Algae such as chlorella in a magnetic field, the intensity of which varies from 4000 to 20 000 oersteds, grow by 106-138% faster than control specimens [18].
The connection between the processes of reprogramming DNA and the intensity of the geomagnetic field has a practical meaning for a living organism. To understand the essence of the phenomenon in this case, it is necessary to look at the problem a little more broadly, referring also to questions of biophysics. All living organisms are capable of supporting homeostasis, that is, of resisting entropy, and this opposition is always associated with energy costs. To do this, different organisms use the energy of chemical bonds or the energy of electromagnetic radiation.
It is known that under certain conditions a DNA molecule has a long half-life. Biologists do not consider virions to be living organisms, but some of them are capable of not destroying for a long time, being in the external environment (tobacco mosaic virus (TMV), green cucumber mosaic (CGMMV), hepatitis). It is believed that DNA molecules in inanimate tissue, as well as virions, do not have the ability to maintain homeostasis, but it is obvious that entropic processes in such complex chemical compounds must occur much more quickly. If we assume that such an ability still exists, then these peculiar organisms must have an energy source for entropy resistance.
To search for such an energy source, I propose to apply the method of elimination. The energy of chemical bonds of DNA can not be used, because chemical reactions will inevitably lead to a change in the genome. Moreover, DNA molecules are better preserved under conditions of isolation from sources of chemical energy, in a purified and dried form. Using the energy of electromagnetic waves is unlikely because of the great instability of such an energy source, and also because of the limitations of the penetrating ability of waves. The most likely source of energy to counteract the growth of entropy due to its constancy, continuity, uniformity of distribution and unlimited penetrating power is the Earth's magnetic field.
To perceive the energy of the geomagnetic field, DNA molecules must have paramagnetic properties. Such properties were discovered by the Soviet biophysicist Lev Blumenfeld. The energy is obtained as follows: the energy of the magnetic field is spent on magnetizing the DNA molecule, and this energy is then released during demagnetization.
In this regard, it is indicative that a prolonged stay of an organism in conditions of a deficit of magnetic energy is the same stress factor and leads to the same negative consequences as the energy deficit, that is, nutrition. Both of these lead to significant disturbances in the homeostasis of animals: a decrease in body weight gain, the predominance of catabolic processes, and differentiation by type of reactivity in assessing interspecific aggression [19]. The geomagnetic field is necessary for the body to maintain homeostasis to the same extent as other types of energy.
Adsorption of the virion does not require energy expenditure, but for the introduction of its RNA (DNA) and enzymes into the living cell - endocytosis, the virion must spend a certain amount of energy, but before the introduction of its RNA and the beginning of the reproductive process, it does not have access to the energy sources of the living cell. It makes sense to assume that the energy of the geomagnetic field is used for endocytosis. It is noted that the multiplication of phages is accelerated during a thunderstorm and during periods of perturbations of the Earth's magnetic field. A number of scientific papers indicate a direct connection between the periods of solar activity and the occurrence of epidemics [20] [21]. From this point of view, it is quite understandable why even a slight change in the intensity of the earth's magnetic field affects the epidemiological situation. It is possible that virions should still be classified as living organisms that have a special way of maintaining homeostasis - due to the energy of magnetic fields.
Let us return to the question of the appropriateness of this property for a living organism. From this point of view, the magnitude of the geomagnetic field strength can be considered as a factor regulating the probability of reprogramming DNA strings, that is, the probability of mutations. The need for such a regulatory mechanism can be justified as follows.
With the strongest solar flares, the geomagnetic field can not completely protect the Earth's atmosphere from the bombardment by charged particles, and this penetrating radiation becomes the most powerful mutagenic factor. Solar storms, in turn, cause and perturbations of the geomagnetic background.
In the initial phase of the geomagnetic storm, the field is amplified, which is caused by the compression of the magnetosphere by the flow of solar plasma. It is during this period of time that the most intense bombardment of the Earth's atmosphere occurs, since particles of higher energies reach our planet earlier. During the period of the main phase of the storm, there is a sharp decline and a sharp restoration of the field, but by this time the flow of high-energy charged particles is already running low.
It can be assumed that this property is a mechanism for protecting gene information during the initial phases of severe geomagnetic storms caused by strong solar flares.
The direct influence of increased solar activity on the epidemiological situation was established reliably. From this point of view, it is also beneficial for the body to stop the processes associated with reprogramming DNA during periods of perturbations of the geomagnetic background.
Unlike solar storms that are short-term in nature, the weakening of the Earth's magnetic field, connected with the polar inversion, is a long process, and can last several thousand years. It has a drastically negative effect on the entire biosphere and this requires accelerating adaptive reactions, that is, on the contrary, increasing the probability of mutations.
In conclusion, I will briefly describe the possible practical application of this mechanism of DNA protection. To increase the shelf life of the genetic material, it should be placed in the gap between two strong permanent magnets striving for connection (this invention is currently being examined by the patent office of the Russian Federation).

1. Mizun Yu. G., Mizun P.G COSMOS AND HEALTH
2. Kaznacheev V. P., Mikhailova L. P. / Bioinformatics function of natural electromagnetic fields. Novosibirsk, 1985
3. Golovin N. A. Kurik M. V., Garnaga N. M. / Biomedical technologies and Radioelectronics. 2002. No. 5-6. P. 41-45
4. Kopanev, V. I., Efimenko G. D., A. V. Sapula / Izv. USSR ACADEMY OF SCIENCES. Ser. Biol. 1979. No. 3. P. 342-346
5. M. L. Kuranova, A. E. Pavlov. I. M. Spivak, S. V. Surma, B. F. Shchegolev. P. A. Kuznetsov, V. E. Stefanov /EFFECTS of HYPOMAGNETIC FIELDS ON LIVING SYSTEMS. Vestnik St. Petersburg University. Ser. 3. 2010. Issue.4. P. 105
6. Lushnikova, E. L., Klinnikova M. T., Young, O. P., Ashheulova N. In. Tissue and intracellular reorganization of the myocardium of mice when exposed to hypogeomagnetic fields/ Bulletin of experimental biology and medicine. 1977. No. 10. P. 455-459
7. Kruglov O. S. investigation of hypomagnetic conditions influence on embryonic development of Japanese quail
8. Asashima M, Shimada K, Pfeiffer CJ. Magnetic shielding induces early developmental abnormalities in the Newt, CYNOPSPYRRHOGASTER. Bioelectromagnetics. 1991; 12(4):215-224. https://doi.org/10. 1002/bem.2250120403 PMID: 1930306
9. Fesenko EE, Mezhevikina LM, Osipenko MA, Gordon RY, Khutzian SS. Effect of the “zero” magnetic field on early embryogenesis in mice. Electromagnetic Biology and Medicine. 2010; 29(1-2):1-8. https://doi.org/10.3109/15368371003627290 PMID: 20230271
10. Wan GJ, Jiang SL, Zhao ZC, Xu JJ, Tao XR, Sword GA, et al. Bio-effects of near-zero magnetic fields on the growth, development and reproduction of small brown planthopper, LAODELPHAXSTRIATELLUS and brown planthopper, NILAPARVATA LUGENS. J Insect Physiol. 2014; 68:7-15. https://doi.org/10.1016/j. jinsphys.2014.06.016 PMID: 24995837
11. Mo WC, Zhang ZJ, Wang DL, Liu Y, Bartlett PF, He RQ. Shielding of the geomagnetic field alters actin assembly and inhibits cell motility in human neuroblastoma cells. Scientific Reports. 2016; 6 (22624):1-15
12. Wang DL, Wang XS, Xiao R, Liu Y, He RQ. Tubulin assembly is disordered in a hypogeomagnetic field. Biochemical and Biophysical Research Communications. 2008; 376(2):363-368. https://doi.org/ 10.1016/j.bbrc.2008.08.156 PMID: 18782559
13. M. Vasilev, R. Dronzin, M. Vasileva, Influence of magnetic field on the process of self-assembly of tubulin, Bolgarskaia Akademiia Nauk 36 (1983) 113-115
14. Belyavskaya NA. Biological effects due to weak magnetic field on plants. Advances in Space Research. 2004; 34(7):1566-1574. https://doi.org/10.1016/j.asr.2004.01.021 PMID: 15880893
15. Bogatina NI, Verkin BI, Litvin VM, Nikulina VF. Effect of weak magnetic fields on Pea growth rate, dry mass, and cell reproduction rate. Doklady of the Academy of Sciences of the Ukrainian SSR, series B —geological, chemical, and biological sciences. 1979;(6):460-463.
16. Ciorba D, Morariu VV. Life in zero magnetic field. III. Activity of aspartate aminotransferase and alanine aminotransferase during in vitro aging of human blood. Electromagnetic Biology and Medicine. 2001; 20(3):313-321
17. Wang X, Xu M, Li B, Li D, Jiang J. Long-term memory was impaired in one-trial passive avoidance task of day-old chicks hatching from hypomagnetic field space. Chinese Science Bulletin. 2003; 48 (22):2454-2457
18. MITCHELL R. SHARPE. LIVING IN SPACE. THE ASTRONAUT AND HIS ENVIRONMENT NEW YORK 1969
19. Features of biotropic action of natural physical factors in the conditions of weakening of the geomagnetic field D. V. Devitsin Novosibirsk 2005
20. Lunchevici D. A., Nikishin, A. I., INFLUENCE of SOLAR ACTIVITY ON the OCCURRENCE of INFLUENZA EPIDEMICS // the Scientific community students: INTERDISCIPLINARY RESEARCH: collection of articles on the Mat. XII international. stud. scientific.- prakt. Conf. No. 1 (12). URL: https://sibac.info/archive/meghdis/1(12)
21. Sukharev V. A. COSMIC NATURE of EPIDEMICS AND PANDEMICS. 2
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Re: Storage of genetic material in a magnetic field

Postby r.rosati » Jun 07 2018 4:57 pm

Soooo... is this for a new bogus "magnetism cure" bracelet?
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