lcdcracker wrote: but I'd like to give it a try, since exchanging buffers with our cleanup kits results in low DNA recovery.
I don't understand what the brand of rest. enzyme has to do with purifying the PCR product?
I thought Fermentas was a reputable company. The biggest RE company in my area resells some of Fermentas's REs that they don't produce themselves.
I have sometimes done a shorcut and successfully digested the PCR product without purifying it from the PCR mix. I don't recommend this. Even if the RE you use is compatable with the conditions of the PCR buffer, prolonged incubation with the PCR polymerase in an exhausted PCR mix, especially if you diluted it, can potentially cause some enzymes to run backwards and have nuclease activity. Commonly available purification kits for PCR products now are very reliable with good yield.
Oh, perhaps the "FastDigest" products are intended to work in the PCR mix? If so, I'd trust them myself if you've had problems purifying. However, this may not be the best diplomatic choice in your current employment situation(?).