background interaction of the probe

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background interaction of the probe

Postby ThilankaMW » Oct 08 2016 3:04 pm

in dna hybridization different annealing reactions can take place one of them is background interaction of the probe is somebody know what is this and how its happen
thank you.
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Re: background interaction of the probe

Postby relaxin » Oct 11 2016 9:02 pm

It is just nonspecific binding of the probe to areas of membrane that do not have DNA bound already and not coated sufficiently with blocking reagents.
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