Annealing temperatures were tested 45-70 degrees.
There were no additional random bands - so I don't think nonspecific binding is the case.
Why would there be more DNA in the 50 and 60 degree, and a sudden decrease in the 55 degree annealing temperature?
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WHY IS 55 DECREASED?
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WHY IS 55 DECREASED?
by sol011 » Oct 31 2016 5:40 pm
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Re: WHY IS 55 DECREASED?
by relaxin » Nov 01 2016 1:37 pm
Is this reproducible? It could be pipetting error.
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