Slightly different DNA storage qestion

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Slightly different DNA storage qestion

Postby Flulol » Mar 15 2017 3:02 pm

Just curious if anyone has advice:

I am looking to see what the best option would be to store a pellet of DNA at room temperature. Part of what I would like to do is ideally have either a dried pellet of genomic DNA which would remain stable at room temperature in a sealed container, or a pellet of genomic DNA in a small sealed container with some other clear solution. Most encountered mol bio questions revolve around dissolving a pellet that is being problematic, I want to find a way to do the opposite. The main concerns for me are relatively long term storage (specifically under these conditions), and prevention or minimization of DNA integrity. I am using a gDNA prep kit that results in approximately 5-12ug of genomic DNA, which is considered ultra clean. Adding other reagents is possible as well. I would potentially like to be able to reverse this (by re cleaning the DNA for downstream apps) but this actually isn't a huge concern. Mainly having a visible DNA pellet that is relatively stable long term. I realize this a weird request.

Also, any other advice for long term DNA storage in solution at room temperature in a small volume would be helpful. I am under the assumption TE would be fine to store gDNA for some time.

Thanks for any advice.
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Re: Slightly different DNA storage qestion

Postby relaxin » Mar 16 2017 3:39 pm

Pure genomic DNA can be stored in a solution in TE at 4 degrees C for a long time. If you want to store it at room temperature, then do ethanol precipitation, rinse with 70% ethanol and dry the pellet. Store the dried pellet in a desiccator.
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