qPCR

Use this category for questions directly related to DNA manipulation (isolation, purification, sequencing, etc.) and questions regarding general PCR methodologies

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qPCR

Postby Thelongwindingcode » Feb 19 2018 4:03 pm

I want to perform qpcr on ssDNA. Do I need to first perform and rt reaction in the same was as starting from RNA?
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Re: qPCR

Postby r.rosati » Feb 19 2018 6:39 pm

No, you don't have to do RT; ssDNA is DNA, and thus it's an adequate template.
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