More info on home recipe for RNase Zap solution

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More info on home recipe for RNase Zap solution

Postby jgjulander » Mar 26 2003 7:31 pm

I found a past post on making a home-made solution of RNase-Zap. There was a recipe including H2O2, SDS and water, but someone else mentioned a better formula using NaOH, SDS and water, but did not give concentrations. Does anyone have a good recipe that works well against RNase?
Thanks,
Justin
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Re: More info on home recipe for RNase Zap solution

Postby AVM » May 06 2003 3:51 pm

jgjulander wrote:I found a past post on making a home-made solution of RNase-Zap. There was a recipe including H2O2, SDS and water, but someone else mentioned a better formula using NaOH, SDS and water, but did not give concentrations. Does anyone have a good recipe that works well against RNase?
Thanks,
Justin


I work with ribosomes, and RNases were always our main concern. Try this mix:

5 % H2O2
0.1 % SDS
0.05 M NaOH

(store at 4C, warm to RT or in 37C water bath and shake before use)

Treat acrylics, plastics and glassware for at least 30 min
Avoid treating metals for over 5-10 min due to corrosion (H2O2 is not good)
Don’t expose to polycarbonate for too long, or avoid it if you can (NaOH is not good)
Rinse off well with autoclaved distilled water after the treatment

Similar solutions, like RNase AWAY, are commercially available and work great!
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