dsRNA purification

Use this category for the exchange of ideas, methodologies and references regarding the isolation, manipulation and analysis of RNA. (Extraction protocols, Northern Blot analysis, RNase Protection, Differential Display, In Vitro Transcription, etc.)

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dsRNA purification

Postby SheilaRNA » Mar 03 2016 7:57 pm

Hi there :D
I´m trying to purified a 187 bp fragment dsRNA expressed in E. coli. I tried acid phenol method (Flegr 1987) but the yield was very low and appeared RNA 28S and 18S. I need dsRNA pure. Please, help me! :cry:
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Joined: Mar 03 2016 7:24 pm

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