In-Vitro assays question

Use this category for the exchange of ideas, methodologies and references regarding the isolation, manipulation and analysis of RNA. (Extraction protocols, Northern Blot analysis, RNase Protection, Differential Display, In Vitro Transcription, etc.)

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In-Vitro assays question

Postby cancerresearcher » Aug 20 2016 8:56 pm

Hi Guys,

I have a question. I was setting up invasion assays in martigel by seeding atrocytes on one side and nothing on other side.And then trying to add neurospheres in the centre of the well.but it didnt work as neurospheres would get stuck in the martigel. What could be other ways to set up invasion assays?

What could I further do to improve my set ups?

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