Immobilizing RNA on the bottom of dish

Use this category for the exchange of ideas, methodologies and references regarding the isolation, manipulation and analysis of RNA. (Extraction protocols, Northern Blot analysis, RNase Protection, Differential Display, In Vitro Transcription, etc.)

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Immobilizing RNA on the bottom of dish

Postby nekameneka » Sep 20 2016 9:56 am

Hi guys,

I want to attach my RNA (or RNA-protein complex) at the bottom of 35mm cell culture dish (glass bottom).
Any suggestions on how should I do this?

Thanks!

:)
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Re: Immobilizing RNA on the bottom of dish

Postby relaxin » Sep 20 2016 3:26 pm

I have no experience on this. You may get some idea from the article below.

http://www.ncbi.nlm.nih.gov/pubmed/15382661
Retired academic researcher. Mention of a specific product does not imply my endorsement of the product. No conflict of interest or guarantee to work on the advice given. Do as I say, not as I do. Not liable to the loss of your valuable samples.
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Re: Immobilizing RNA on the bottom of dish

Postby nekameneka » Sep 21 2016 1:33 am

Thanks a lot for the link, I missed this one somehow when I was searching. :D
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