RNA-RNA Gel mobility shift assay(EMSA)

Use this category for the exchange of ideas, methodologies and references regarding the isolation, manipulation and analysis of RNA. (Extraction protocols, Northern Blot analysis, RNase Protection, Differential Display, In Vitro Transcription, etc.)

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RNA-RNA Gel mobility shift assay(EMSA)

Postby hasmat buchad » Dec 17 2016 7:49 am

hello everyone

I am doing sRNA-mRNA EMSA. I labeled my sRNA with digoxygenin and elute from urea-page. labeled sRNA with increasing unlabelled mRNA gave some shifted band of higher size. I also perform the same experiment with unlabelled sRNA and mRNA and stained gel with ethidium bromide but no higher shift was observed. I don't know which one to consider correct.
Kindly help
hasmat buchad
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