ATP and its analogues

Use this category for questions regarding problems manipulating proteins in molecular biology applications (expression, detection, etc.)

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ATP and its analogues

Postby nekameneka » Mar 03 2018 10:23 am

Hi everyone,
I have been searching around and would like to ask you here an explanation for some of the ATP roles used in nucleic acid-protein experiments.
Can someone tell me more about "intermediate" state ADP-AlFx? I understand that AMP-PNP analogue is non-hydrolyzable and represents the "bound" state.
I would like to know what ADP-AlFx represents in the meaning of nucleic acid-protein interaction? What does "intermediate" actually means here?

Thank you very much!
nekameneka
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