Lysis buffer

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Lysis buffer

Postby newbie17425 » Nov 16 2016 7:36 pm

My proteins of interest are inside the cell membrane. Is there any specific lysis buffer or can I use RIPA buffer to lyse my tissues. My tissue of interest is the hippocampus.

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Re: Lysis buffer

Postby relaxin » Nov 17 2016 5:03 pm

For Western blot, RIPA plus protease inhibitor cocktail will be fine.
Retired academic researcher. Mention of a specific product does not imply my endorsement of the product. No conflict of interest or guarantee to work on the advice given. Do as I say, not as I do. Not liable to the loss of your valuable samples.
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