Mass spec

Use this category for questions regarding problems manipulating proteins in molecular biology applications (expression, detection, etc.)

Moderators: mdfenko, leekaming

Mass spec

Postby TinaRina19 » Jan 11 2017 3:04 pm

Hello,
I have to come up with a research project for university and I am planning to find possible binding partners of a specific signalling molecule in Drosophila. I have an antibody against the protein so I can co-precipitate the protein and its binding partners. Now my question, can I do the usal MALDI-TOF or would the data too hard to anaylse since there might be many mayn binding partners. Should I purify it before with a 2D gel to get results that I can easier interprete?

Thanks in advance
Tina
TinaRina19
newcomer
newcomer
 
Posts: 1
Joined: Jan 11 2017 2:59 pm

Return to Protein Methods

Who is online

Users browsing this forum: No registered users and 1 guest