Biotin Assay Controls

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Biotin Assay Controls

Postby roymiller11 » Feb 22 2017 7:03 pm

I am performing a biotin assay to quantify protein in bone marrow derived macrophages over a 24 hour period in intervals of 3 hours.

I am seeking a good control to compare quantity to, however both actin and tubulin do not appear on my western blots.
The control must have a known half-life and be able to be detected under the biotin assay.

Thank you for your suggestions,
Roy Miller
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Re: Biotin Assay Controls

Postby mdfenko » Feb 23 2017 12:21 pm

i'm not sure what you mean by "biotin assay".

biotin binds exclusively with avidin and streptavidin. it wouldn't directly bind to actin or tubulin.

if you would give us specific information as to how you perform your "assay",, then we may be able to guide you.
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