Magnetic bead separation vs Sepharose

Use this category for questions regarding problems manipulating proteins in molecular biology applications (expression, detection, etc.)

Moderators: mdfenko, leekaming

Magnetic bead separation vs Sepharose

Postby newbie17425 » Feb 11 2018 11:39 pm

Hi,

I am doing coIP and currently using Sepharose beads for my experiments from GE (https://www.gelifesciences.com/shop/chr ... t=17088501)

I also found that some people use magnetic beads for separation of the proteins. For example, https://www.thermofisher.com/order/cata ... duct/88804

Can you suggest what is the most efficient separation method and what is the advantage of magnetic beads over sepharose beads?

Thanks
newbie17425
Prolific Post-Master
Prolific Post-Master
 
Posts: 138
Joined: May 22 2015 8:23 pm

Re: Magnetic bead separation vs Sepharose

Postby relaxin » Feb 13 2018 4:21 pm

I have used magnetic beads. The advantage is that they can be pulled down with magnet, which is faster than centrifugation. The volume is smaller than the beads (less trapped protein solution). This will facilitate washing of the immunoprecipitate.
Retired academic researcher. Mention of a specific product does not imply my endorsement of the product. No conflict of interest or guarantee to work on the advice given. Do as I say, not as I do. Not liable to the loss of your valuable samples.
relaxin
PI of Posters
PI of Posters
 
Posts: 7189
Joined: Jan 11 2006 5:40 pm
Location: Mauna Kea


Return to Protein Methods

Who is online

Users browsing this forum: No registered users and 3 guests