Phage display library

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Phage display library

Postby chisie2009 » Dec 25 2016 3:35 pm

Hello,

There is a step that I don't understand when I read phage display library construction protocols.

If I understand correctly, after transformation, each colony on the agar plate represents a clone in the final library.
But phage display libraries are very large in the range of 10e8 - 10e10 clones. If one agar colony represents one clone, how do you prepare a library of such a huge amount of colonies?
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Re: Phage display library

Postby relaxin » Dec 26 2016 1:04 pm

The phage library is constructed as a mixture. You have to screen the colonies at high density and then rescreen at low density to find the ones you want.
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