IHC resolution

Use this category for questions regarding various immunological methods

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IHC resolution

Postby ajmick » Jan 14 2016 10:07 am

I'm having a recurring issue with my IHC stainings looking unfocused and of bad quality with the 20x objective. The staining works, however the pictures are coming out really bad. We have determined it is not a microscope issue, by taking pictures of a colleague's IHC in the same session as my stainings. Does anyone know what can be causing this? I've also attached a picture as an example.
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