ELISA with MW2:mixing with antibiotic and honey

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ELISA with MW2:mixing with antibiotic and honey

Postby Mimi9417 » Mar 10 2016 10:04 pm

I am trying to come up with a method for an ELISA using MW2 (MRSA strain). The MW2 culture will be mixed with an antibiotic and manuka honey to see if PBP2A is inhibited. I have found basic protocols, but I am unsure on what specific antibody to use in order to ultimately view PBP2A's production. Any help and/or suggestions on possible methods would be much appreciated! Thank you!
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Re: ELISA with MW2:mixing with antibiotic and honey

Postby mdfenko » Mar 11 2016 7:30 pm

have you located a commercial source of antibody against pbp2a?

if so, the description should tell you for which procedures the antibody will work.

if not, then you'll have to either find a commercial source or a lab that prepares its own or produce it yourself.

if another lab or yours produces the antibody then it will have to have been produced against the native protein or a peptide of an exposed (in the native form) epitope.
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