Hybridoma Technology

Use this category for questions regarding various immunological methods

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Hybridoma Technology

Postby dwaggoner » Oct 20 2016 2:00 pm

Hi,
Is there a reason as to why NOT include circulating blood cells (in addition to isolated splenocytes) in a fusion event with plasmacytomas when trying to generate a mouse mAb against a difficult antigen (haptenized small molecule, for example)?....If appropriate numbers of plasmacytomas are used? Is it simply a numbers game with regard to successful fusion frequency (numbers of colonies generated in a fusion reaction)? Or is there a viable argument that plasma cells (against the antigen of interest) are not circulating, or that plasmablasts (against the antigen of interest) present in the circulation result in genetically unstable hybridomas? Other reasons?
David
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