Exam revision - Practice questions

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Exam revision - Practice questions

Postby biologyegn » Apr 06 2017 4:14 pm

Hello everyone!

I have an impending exam next month and my lecturers have given us a bank of various questions (75 in total) to help with revision.

I'm struggling with a couple of them, so any help and advice would be greatly appreciated!

They are as follows;

The length of your PCR product is determined by:

a. The distance between the two primer binding sites and including half of the length of the two primer sequences

b. The distance between the two primer binding sites, not including the entire lengths of the two primer sequences

c. The lengths of the two primers added together

d. The distance between the two primer binding sites, including the entire lengths of the two primer sequences

Eukaryotic DNA is wrapped around 4 histone proteins to form a nucleosome it is further condensed into a chromatin structure. In order for the chromatin structure to unwind, to activate genes in particular regions, the following two things must happen:

a. The DNA sequence for the particular gene must first be methylated and then the Histone proteins must be acetylated. The DNA must then be unwound in order that RNA polymerase can bind to the template anti-sense strand to transcribe the gene sequence.

b. The DNA sequence for the particular gene must first be demethylated and then the Histone proteins must be acetylated. The DNA must then be unwound in order that RNA polymerase can bind to the template anti-sense strand to transcribe the gene sequence.

c. The DNA sequence for the particular gene must first be demethylated and then the Histone proteins must be deacetylated. The DNA must then be unwound in order that DNA polymerase can bind to the template anti-sense strand to transcribe the gene sequence.

d. The DNA sequence for the particular gene must first be demethylated and then the Histone proteins must be deacetylated. The DNA must then be unwound in order that RNA polymerase can bind to the template anti-sense strand to transcribe the gene sequence.


The annealing temperature in a PCR reaction is usually approximately 5 oC, below the lowest Tm of your two PCR primers:

a. To allow approximately 100% binding of both of your primers to specifically bind to their intended targets on the genomic DNA.

b. To allow approximately 50% binding of both of your primers to specifically bind to their intended targets on the genomic DNA.

c. To allow approximately 100% binding of both of your primers to each other.

d. To allow approximately 50% binding of both of your primers to each other.

A typical human gene consists of:

a. A promoter region, exons, which contain both coding sequence and untranslated sequence, and introns which, consist of untranscribed sequence.

b. A promoter region exons, which only contain coding sequence and introns which, consist of transcribed, but not translated sequence.

c. A promoter region exons which only contain coding sequence and introns which, consist of untranscribed sequence

d. A promoter region, exons, which contain both coding sequence and untranslated sequence, and introns which, consist of transcribed but not translated sequence.




Thank you very much in advance for any help with the questions!
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Re: Exam revision - Practice questions

Postby r.rosati » Apr 06 2017 4:26 pm

Hello!
To start the discussion, what are your thoughts on these questions so far? We can help if we know how to direct you to find more information, but people are unlikely to just outright do homework for you.
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